学    历

1995, 9-1999, 6 华中农业大学 微生物学 博士

职    历

1993, 6-现在 华中农业大学生命科学技术学院/农业微生物学国家重点实验室;

2000, 7－2002，4, 在韩国Pohang University of Science and Technology化工系访问学者;

2003, 1－2004, 12, 德国Max-Planck-Institut fuer InfeksBiologies博士后

研究方向

以假单胞菌、芽孢杆菌、大肠杆菌和谷氨酸棒杆菌等原核细菌和酵母菌为对象，主要进行环境降解、底盘细胞改造、功能元件研发与适配性研究、基因表达活性调控、作用机制与应用技术基础等方面的研究工作。目前主要科研方向为：

（1）假单胞菌降解环境难降解和有毒化合物的功能基因、降解活性、作用途径与机制；

（2）运用CRISPR-Cas9/CRISPR-Cas12f1、CRISPR-RNA等基因编辑工具进行假单胞菌、芽孢杆菌、大肠杆菌和谷氨酸棒杆菌基因组简约编辑，规模性删除基因组冗余基因和改造细菌底盘细胞；

（3）体内连续进化并联合体外进化策略，以理性设计、半理性设计和构建自组装人工多酶复合体等生物技术策略实现对不同酶蛋白的分子改造，以大幅提升酶催化活性与酶底物适配性；

（4）针对细菌和酵母细胞工厂不同元件的适配性研究。已在假单胞菌、大肠杆菌和酵母菌成功发掘不同强度的系列启动子、RBS、群体感应系统单元和菌体自杀系统单元，以适应于不同使用目的需要。

主要兼职

曾担任中国微生物学会普通微生物专业委员会副主任

电    话

027-8728 6952 (O)

电子邮件

lilin@mail.hzau.edu.cn

工作单位

华中农业大学生命科学技术学院/农业微生物资源发掘与利用全国重点实验室/微生物农药国家工程研究中心

研究成果

先后主持国家863项目1项、国家973项目课题1项、国家自然科学基金重点项目课题2项和国家自然科学基金面上项目7项，以及主持省部级和横向各类项目20余项。发表SCI论文80余篇，其它学术论文30余篇，参与撰写英文学术专著2部，中文学术专著2部，获的授权国家发明专利7项。参与获得湖北省技术发明一等奖、农业部科技进步三等奖和武汉市科技进步二等奖等奖励。

   近5年发表的代表性论著（*通讯作者）

[1] Yongxuan Liu, Jiaoqing Li, Jie Zeng, Xun Yu, Xiaowen Sun, Zhicheng Zhou, Jingjing Xu, Liangzheng Xu, Lin Li*. Complete oxidative degradation of diclofenac via coupling free radicals and oxygenases of a micro/nanostructured biogenic Mn oxide composite from engineered Pseudomonas sp. MB04R-2. Journal of Hazardous Materials 2023, 456: 131657.

[2] Xiaowen Sun, Zhe Li, Jiaoqing Li, Zhi Li, Yini Ma, Zhicheng Zhou, Yongxuan Liu, Jie Zeng, Liangzheng Xu, Lin Li*. Dynamic composting actuated by a Caldibacillus thermoamylovorans isolate enables biodecomposability and reusability of Cinnamomum camphora garden wastes. Bioresource Technology 2023, 376: 128852.

[3] Asmaa Sh. M. Daoush, Mohamed H. Hendawey, Rabaa Yaseen, Ahmed S. M. El-Nuby, Tarek M. Bedair, Khairiah Mubarak Alwutayd, Nawal Al-Hoshani, Ahmed Shaaban, Anum Bashir, Lin Li*. Effect of biosynthesized nanoselenium on controlling tomato root-knot nematode Meloidogyne incognita. Agronomy 2023, 13: 1668.

[4] Muhammad Ali, Tong Gu, Xun Yu, Anum Bashir, Zhiyong Wang, Xiaowen Sun, Naeem Mahmood Ashraf, Lin Li*. Identification of the genes of the plant pathogen Pseudomonas syringae MB03 required for the nematicidal activity against Caenorhabditis elegans through an integrated approach. Frontiers in Microbiology 2022, 13: 826962.

[5] Tan Wang, Kexin Yang, Qingyun Ma, Xu Jiang, Yiqing Zhou, Delong Kong, Zhiye Wang, Rebecca E. Parales, Lin Li*, Xin Zhao*, Zhiyong Ruan*. Rhizosphere microbial community diversity and function analysis of cut chrysanthemum during continuous monocropping. Frontiers in Microbiology, 2022, 13: 801546.

[6] Tong Gu, Zhenghu Tong, Xue Zhang, Zhiyong Wang, Zhen Zhang, Tzann-Shun Hwang*, Lin Li*. Carbon metabolism of a soilborne Mn(II)-oxidizing Escherichia coli isolate implicated as a pronounced modulator of bacterial Mn oxidation. International Journal of Molecular Sciences, 2022, 23: 5951.

[7] Anum Bashir, Huafu Yu, Abdul Manan, Lin Li*. Nematicidal effects of a peptidase (NlpC/P60) from the phytopathogen Pseudomonas syringae MB03 on the model nematode Caenorhabditis elegans and plant-parasitic Meloidogyne incognita. Molecular Biology Reports, 2022, 49: 6313-6324.

[8] Xun Yu, Cui Meng, Xiaocheng Tan, Yuwei Su, Zhiping Cao, Tzann-Shun Hwang, Lin Li*. RsmA3 modulates RpoS through the RetS-Gac-Rsm signaling pathway in response to H₂O₂ stress in the phytopathogen Pseudomonas syringae. Environmental Microbiology, 2022, 24: 4755-4770.

[9] Jiaoqing Li, Meijuan Ding, Xiaowen Sun, Zhe Li, Liangzheng Xu*, Lin Li*. Characterization of nematicidal activity and nematode-toxic metabolites of a soilborne Brevundimonas bullata isolate. Pathogens, 2022, 11, 708.

[10] Jin Liu, Tong Gu, Li Li, Lin Li*. Synthesis of MnO/C/NiO-doped porous multiphasic composites for lithium-ion batteries by biomineralized Mn oxides from engineered Pseudomonas putida cells. Nanomaterials 2021, 11: 361.

[11] Xiaowen Sun, Run Zhang, Meijuan Ding, Yongxuan Liu, Lin Li*. Biocontrol of the root-knot nematode Meloidogyne incognita by a nematicidal bacterium Pseudomonas simiae MB751 with cyclic dipeptide. Pest Management Science 2021, 77: 4365-4374.

[12] Jin Liu, Tong Gu, Xiaowen Sun, Li Li, Fan Xiao, Zhiyong Wang, Lin Li*. Synthesis of MnO/C/Co₃O₄ nanocomposites by a Mn²⁺-oxidizing bacterium as a biotemplate for lithium-ion batteries. Science and Technology of Advanced Materials 2021, 22(1): 429-440.

[13] Li Li, Jin Liu, Jie Zeng, Jiaoqing Li, Yongxuan Liu, Xiaowen Sun, Liangzheng Xu*, Lin Li*. Complete degradation and detoxification of ciprofloxacin by a Micro-/nanostructured biogenic Mn oxide composite from a highly active Mn²⁺-oxidizing Pseudomonas strain. Nanomaterials 2021, 11: 1660.

[14] Anum Bashir, Yu Sun, Xun Yu, Xiaowen Sun, Lin Li*. Nematicidal effects of 2-methyl-aconitate isomerase from the phytopathogen Pseudomonas syringae MB03 on the model nematode Caenorhabditis elegans. Journal of Invertebrate Pathology 2021, 185: 107669.

[15] Anum Bashir, Tian Tian, Xun Yu, Cui Meng, Muhammad Ali, Lin Li*. Pyoverdine-mediated killing of Caenorhabditis elegans by Pseudomonas syringae MB03 and the role of iron in its pathogenicity. International Journal of Molecular Sciences. 2020, 21: 2198.

[16] Zhiqiang Bai, Xiaowen Sun, Xun Yu, Lin Li*. Chitosan microbeads as supporter for Pseudomonas putida with surface displayed laccases for decolorization of synthetic dyes. Applied Sciences 2019, 9, 138.

[17] Zhen Zhang, Zhiyong Ruan, Jin Liu, Chang Liu, Fuming Zhang, Robert J. Linhardt, Lin Li*. Complete degradation of bisphenol A and nonylphenol by a composite of biogenic managanese oxides and Escherichia coli cells with surface-displayed multicopper oxidase CotA. Chemical Engineering Journal 2019, 362: 897-908.

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