何正国,教授,博士生导师

教育经历

1988-1995 华中农业大学生命科学技术学院微生物专业学士、硕士学位

1998-2001 中科院微生物所微生物学专业博士学位

科研与学术工作经历

2001-2004 哈佛医学院生物化学与分子药物系博士后

2004-2005 湖北大学生命科学学院教授

2005- 今 华中农业大学生命科学技术学院教授、博士生导师

农业微生物学国家重点实验室PI

研究方向

病原细菌学、感染与免疫、抗菌疫苗设计

代表性研究成果（*为通讯作者）

1.Yu Liu, Hongyang Wang, Tao Cui, Xiling Zhou, Yanxia Jia, Hua Zhang*, Zheng-Guo He* (2016) NapM, a new nucleoid-associated protein, broadly regulates gene expression and affects mycobacterial resistance to anti-tuberculosis drugs. Mol Microbiol.101(1): 167-181.

2. Weihui Li^#, Tao Cui^#, Lihua Hu^#, Ziqing Wang, Zongqiang Li, Zheng-Guo He* (2015) Cyclic diguanylate monophosphate directly binds to human siderocalin and inhibits its antibacterial activity. Nat Commun. 6: 8330.

3. Min Yang, Chun-Hui Gao, Jialing Hu, Lei Zhao, Qiaoyun Huang, Zheng-Guo He* (2015) InbR, a TetR family regulator, binds with isoniazid and influences multidrug resistance in Mycobacterium bovis BCG. Sci Rep.5: 13969

4. Tao Cui, Zheng-Guo He* (2014) Improved understanding of pathogenesis from protein interactions in Mycobacterium tuberculosis. Expert Rev Proteomics 11(6):745-755.

5. Min Yang, Chunhui Gao, Jialing Hu, Chao Dong, Zheng-Guo He* (2014)Characterization of the interaction between a SirR family transcriptional factor of Mycobacterium tuberculosis, encoded by Rv2788, and a pair of toxin-antitoxin proteins RelJ/K, encoded by Rv3357 and Rv3358. FEBS J.281(12): 2726-2737

6. Lei Liu, Cheng Huang, Zheng-Guo He*. (2014)A TetR-family transcriptional factor directly regulates the expression of a 3-methyladenine DNA glycosylase and physically interacts with the enzyme to stimulate its base excision activity in Mycobacterium bovis BCG. J Biol Chem. 289(13):9065-9075

7. Hua Zhang, Zhuanmei Zhang, Jian Yang, Zheng-Guo He* (2014)Functional characterization of DnaB helicase and its modulation by single-stranded DNA-binding protein in Mycobacterium tuberculosis. FEBS J. 281: 1256-1266

8. Shifan Yang^#, Zengqiang Gao^#, Tingting Li, Min Yang, Tianyi Zhang, Yuhui Dong*, Zheng-Guo He* (2013)Structural basis for interaction between M. smegmatis Ms6564, a TetR family master regulator, and its target DNA. J Biol Chem. 288(33): 23687-95.

9. Lei Zhang, Zheng-Guo He* (2013)Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect its cyclic-di-AMP synthesis activity in Mycobacterium smegmatis.J Biol Chem. 288(31): 22426-22436

10. Lei Zhang, Weihui Li, Zheng-Guo He* (2013)DarR, a TetR-like transcriptional factor, is a cyclic-di-AMP responsive repressor in Mycobacterium smegmatis. J Biol Chem. 288 (5): 3085-3096

11. Muding Rao^#, Huicong Liu^#, Min Yang, Chunchao zhao, Zheng-Guo He* (2012)A copper-responsive global repressor regulates expression of diverse membrane-associated transporters and bacterial drug resistance in mycobacteria. J Biol Chem. 287(47): 39271-39371

12. Weihui Li, Zheng-Guo He* (2012)LtmA, a novel cyclic di-GMP responsive activator, broadly regulates the expression of lipid transport and metabolism genes in Mycobacterium smegmatis. Nucleic Acids Res. 40(22): 11292-307

13. Jumei Zeng^#, Tao Cui^#, Zheng-Guo He* (2012)A Genome-wide Regulator-DNA Interaction Network in the human pathogen Mycobacterium tuberculosis H37Rv. J Proteome Res. 11(9): 4682-4692

14. Min Yang, Chunhui Gao, Tao Cui, Jingning An, Zheng-Guo He* (2012) A TetR-like regulator broadly affects the expressions of diverse genes in Mycobacterium smegmatis. Nucleic Acids Res. 40(3): 1009-1020

15. Jumei Zeng, Yuqing Li, Shuguang Zhang, Zheng-Guo He* (2012) A novel high-throughput B1H-ChIP method for efficiently validating and screening specific regulator-target promoter interactions. Appl Microbiol Biotech. 93: 1257–1269

16. Yi Wang^#, Yuanxia Huang^#, Chaolun Xue, Yang He, Zheng-Guo He* (2011) A ClpR-like regulator specifically recognizes a RecA-independent promoter motif and broadly regulates expression of DNA damage inducible genes in mycobacteria. J Biol Chem. 286(36): 31159-31167.

17.Qiong Yang, Yuanyuan Liu, Feng Huang, Zheng-Guo He* (2011) Physical and functional interaction between D-ribokinase and topoisomerase I has opposite effects on their respective activity in M. smegmatis and M. tuberculosis Arch Biochem Biophys. 512: 135-142

18. Yi Wang^#, Tao Cui^#, Cong Zhang, Min Yang, Yuanxia Huang, Weihui Li, Lei Zhang, Chunhui Gao, Yang He, Yuqing Li, Feng Huang, Jumei Zeng, Cheng Huang, Qiong Yang, Yuxi Tian, Chunchao Zhao, Huanchun Chen, Hua Zhang, Zheng-Guo He* (2010) Global protein-protein interaction network in the human pathogen Mycobacterium tuberculosis H37Rv. J Proteome Res. 9(12): 6665 - 6677.

19.Yuqing Li, Jumei Zeng, Hua Zhang, Zheng-Guo He* (2010)The characterization of conserved binding motifs and potential target genes for M. tuberculosis MtrAB reveals a link between the two-component system and the drug resistance of M. smegmatis. BMC Microbiol. 10(1): 242

20. Feng Huang, Zheng-Guo He* (2010) Characterization of an interplay between a Mycobacterium tuberculosis MazF homolog, Rv1495, and its sole DNA topoisomerase I. Nucleic Acids Res.38(22): 8219-8230

21.Yuqing Li, Jumei Zeng, Jianfang Shi, Mingchao Wang, Muding Rao, Chaolun Xue, Yanli Du, Zheng-Guo He* (2010) A proteome-scale identification of novel antigenic proteins in Mycobacterium tuberculosis towards diagnostic and vaccine development. J Proteome Res. 9(9): 4812 - 4822

22. Bin Wang, Shifan Yang, Lei Zhang, Zheng-Guo He*（2010）Archaeal eukaryote-like serine/threonine protein kinase interacts with and phosphorylates a forkhead-associated domain-containing protein. J Bacteriol. 192(7): 1956-1964.

23. Lu Zhang^#, Lei Zhang^#, Yi Liu^#, Shifan Yang, Chunhui Gao, Hongchao Gong, Ying Feng, Zheng-Guo He* (2009) Archaeal eukaryote-like Orc1/Cdc6 initiators physically interact with DNA polymerase B1 and regulate its functions. Proc Natl Acad Sci USA. 106(19): 7792-7797.

24. Manman Guo, Hui Feng, Jun Zhang, Wenqin Wang, Yi Wang, Yuqing Li, Chunhui Gao, Huanchun Chen, Ying Feng, Zheng-Guo He* (2009) Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 19(7): 1301-1308.

25. Tao Cui, Lei Zhang, Xizhou Wang, Zheng-Guo He* (2009) Uncovering new signaling proteins and potential drug targets through the interactome analysis of Mycobacterium tuberculosis. BMC Genomics. 10(1):118

主讲课程

微生物功能基因组学

研究生招生专业

微生物学

联系方式

实验室电话：027-87284295

电子邮箱：hezhengguo@mail.hzau.edu.cn

办公地点：农业微生物学国家重点实验室（外三楼2楼201）