分子生物学教研室Brief Introduction

彭东海
时间:2015-11-03

 

 

 

彭东海,博士,副教授,硕士生导师

教育经历(从大学本科开始)

2000-2004 华中农业大学生命科学技术学院生物技术专业理学学士学位

2004-2009 华中农业大学生命科学技术学院微生物学专业理学博士学位

 

科研与学术工作经历

2017/06—2017/12,美国康奈尔大学,访问学者

2013/01—至今, 华中农业大学,生命科学与技术学院,副教授

2009/12—2010/02,英国苏赛克斯大学,访问学者

2009/07—2012/12,华中农业大学,生命科学与技术学院,讲师

 

研究方向

微生物农药,病原细菌与宿主相互作用

 

主持科研项目情况:

1.    国家自然科学基金青年项目(31000020),苏云金芽胞杆菌溶源性噬菌体裂解关键控制因子,2011/01-2013/1219万元,主持;

2.    国家973项目(2013CB1275),芽胞杆菌侵染线虫的分子机制,2013/01-2017/12100.0万元,子课题主持;

3.    横向课题,珠海冬种蔬菜软腐病和黄曲条跳甲生物防治的技术研究和应用示范,2014/01-2015/125.0万元,主持;

4.    国家自然科学基金面上项目(31370002),苏云金芽胞杆菌控制溶源性噬菌体关键因子的功能和作用模式,2014/01-2017/1280万元,主持;

5.    中央高校基本科研业务费(2662016py067),苏云金芽胞杆菌与其噬菌体的相互作用,2016/01-2019/0118万元,主持;

6.    国家重点研发计划项目(31370002),苏云金芽胞杆菌高效杀线虫机制研究,2017/04-2020/1275万元,子课题主持;

7.    国家自然科学基金面上项目(31370002),苏云金芽胞杆菌控制溶源性噬菌体关键因子的功能和作用模式,2018/01-2021/1265万元,主持。

 

代表性研究成果

1.    Peng DH, Luo XX, Zhang N, Guo SX, Zheng JS, Chen L, Sun M*. (2017) Small RNA−mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavior defenses. Nucleic Acids Res., Epub, doi: 10.1093/nar/gkx959.

2.    Geng C#, Nie XT#, Tang ZC, Zhang YY, Lin J, Sun M, Peng DH*. (2016) A novel serine protease, Sep1, from Bacillus firmus DS-1 has nematicidal activity and degrades multiple intestinal-associated nematode proteins. Sci Rep, 6:25012.

3.    Peng DH, Lin J, Hang Q, Zheng W, Liu GQ, Zheng JS, Zhu L, Sun M*. (2016) A novel metalloproteinase virulence factor is involved in B. thuringiensis pathogenesis in nematodes and insects. Environ Microbiol, 18(3):846-862.

4.    Zhang FJ, Peng DH, Cheng CS, Zhou W, Ju SY, Wan DF, Yu ZQ, Shi JW, Deng Y, Wang F, Ye XB, Hu ZF, Lin J, Ruan LF, Sun M*. (2016) Bacillus thuringiensis Crystal Protein Cry6Aa Triggers Caenorhabditis elegans Necrosis Pathway Mediated by Aspartic Protease (ASP-1). PLoS Pathog. 12(1): e1005389.

5.    Zheng JS, Peng DH, Chen L, Liu HL, Chen F, Xu MC, Ju SY, Ruan LF, Sun M*. (2016) The Ditylenchus destructor genome provides new insights into the evolution of plant parasitic nematodes. Proc Biol Sci. 286(1835).

6.    Peng DH, Pang CY, Wu H, Huang Q, Zheng JS, Sun M*. (2015) The expression and crystallization of Cry65Aa C-termini, revealing a novel evolutionary strategy of Bacillus thuringiensis Cry proteins. Sci Rep, 5: 8291.

7.    Ruan LF, Crickmore N, Peng DH, Sun M. (2015) Are nematodes a missing link in the confounded ecology of the entomopathogen Bacillus thuringiensis? Trends Microbiol. 23(6):341-346.

8.    Zhang FJ#, Peng DH#, Ye XB, Yu ZQ, Hu ZF, Ruan LF, Sun M*. (2012) In vitro uptake of 140 kDa Bacillus thuringiensis nematicidal crystal proteins by the second stage juvenile of Meloidogyne hapla. PLoS One, 7: e38534.

9.    Peng DH, Wang FS, Li NS, Zhang ZY, Song R. Zhu ZM, Ruan LF, Sun M*. (2011) Single cysteine substitution in Bacillus thuringiensis Cry7Ba1 improves the crystal solubility and produces toxicity to Plutella xylostella larvae. Environ Microbiol, 13:2820-2831.

10. Peng DH, Qiu DW, Ruan LF, Zhou CF, Sun M*. (2011) Protein elicitor PemG1 from Magnaporthe grisea induces SAR in plants through the salicylic acid and Ca-related signaling pathways. Mol Plant Microbe Interact, 24:1239-1246.

11. Peng DH, Chai LJ, Wang FS, Zhang FJ, Ruan LF, Sun M*. (2011) Synergistic activity between Bacillus thuringiensis Cry6Aa and Cry55Aa toxins against Meloidogyne incognita. Microb Biotechnol, 4(6):794-798.

12. Zhong CY#, Peng DH#, Ye WX, Chai LJ, Qi JL, Yu ZN, Ruan LF, Sun M*. (2011) Determination of plasmid copy number reveals the total plasmid DNA amount is greater than the chromosome DNA amount in Bacillus thuringiensis YBT-1520. PLoS One, 6: e16025.

13. Zhou Z#, Peng DH#, Zheng J, Guo G, Tian L, Yu Z, Sun M. (2011) Two groups of S-layer proteins, SLP1s and SLP2s, in Bacillus thuringiensis co-exist in the S-layer and in parasporal inclusions. BMB Rep. 44(5):323-328.

14. Peng DH#, Xu XH#, Ye WX, Yu ZN, Sun M*. (2010) Helicoverpa armigera cadherin fragment enhances Cry1Acinsecticidal activity by facilitating toxin-oligomer formation. Appl Microbiol Biotechnol, 85:1033-1040.

15. Peng DH, Xu XH, Ruan LF, Yu ZN, Sun M*. (2010) Enhancing Cry1Actoxicity by expression of the Helicoverpa armigera cadherin fragment in Bacillus thuringiensis. Res Microbiol, 161:383-389.

16. Peng DH, Luo Y, Guo SX, Zeng H, Ju SY, Yu ZN, Sun M*. (2009) Elaboration of an electroporation protocol for larger plasmids and wild-type strains in Bacillus thuringiensis. J Appl Microbiol, 106:1849-1858.

17. Peng DH, Zhou CF, Chen SW, Ruan LF, Yu ZN, Sun M*. (2008) Toxicological safety assessment of genetically modified Bacillus thuringiensis with additional N-Acyl homoserine lactonase gene. Environ Toxicol Chem, 27: 173-180.

18. Peng DH, Chen SW, Ruan LF, Li L, Yu ZN, Sun M*. (2007) Safety assessment of transgenic Bacillus thuringiensis with VIP insecticidal protein gene by feeding studies. Food Chem Toxicol, 45, 1179-1185.

论文清单见NCBI网站: http://www.ncbi.nlm.nih.gov/pubmed/?term=Peng+D%2C+Sun+M 

 

获奖情况

2015年指导IGEM团队获得国际遗传工程机器设计大赛金奖;

2015年荣获华中农业大学2015学科竞赛优秀指导教师

201320142016年获华中农业大学教学质量优秀三等奖;

20112015年被评选为湖北省优秀学士学位论文指导教师。

 

主讲课程

基因操作原理

 

研究生招生专业

微生物农药,噬菌体

 

联系方式

实验室电话:027-87283455

电子邮箱:donghaipeng@mail.hzau.edu.cn

办公地点:农业微生物学国家重点实验室A609

 

实验室介绍及网址:

芽胞杆菌分子生物学(BMB)实验室

http://bmb.hzau.edu.cn/

 

中国·湖北·武汉 南湖狮子山街一号430070 Tel:+86-27-87282101 Fax:+86-27-87282469
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