Xingwang Li（李兴旺）
Professor
National Key Laboratory of Crop Genetic Improvement
College of Life Science and Technology
Huazhong Agricultural University
Wuhan 430070, China.
Research
The linear genome of higher organisms are known to be folded into chromosomal territories within the three-dimensional (3D) nuclear. Recent achievements on 3D genome organization have revealed frequent chromatin loops within topological-associated domains (TADs) and have implicated general mechanism of chromatin loops between gene promoters and enhancers for transcriptional regulation. Rice (Oryza sativa) is one of the most important food crops in the world and has been established as a model plant for biological research. To explore 3D genome structures and its dynamic (4D) in rice, we have been focused on developing plants 3D genome mapping technologies and exploring mechanism of 3D genome structure on transcription regulation since 2013. More specifically, we applied cutting-edge 3D genome mapping technologies and characterized genome-wide DNA-DNA, RNA-DNA interactions mediated by active gene promoters and heterochromatin at various developmental stages and examined the comprehensive hierarchical 3D genome structure and their dynamics as well as their impacts on transcriptional regulation.
Education
2003-09 to 2011-06: PhD in Biochemistry and Molecular Biology, Huazhong Agricultural University, Wuhan, China, Supervisor: Qifa Zhang, Changyin Wu
1999-09 to 2003-07: B.S. Biotechnology, Inner Mongolia Agricultural University, Huhhot, China
Research and work experience
2016-08 to present：Professor in Genomcis, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China
2013-07 to 2016-07：Post Doctorate, The Jackson Laboratory for Genomic Medicine, Connecticut, USA
2011-07 to 2013-06: Research Associate, National Key Laboratory of Crop Genetic Improvement, Wuhan, China
Representative publications
1. Qin Xiao*, Xingyu Huang*, Yan Zhang, Wei Xu, Yongqing Yang, Qing Zhang, Zhe Hu, Feng Xing, Qianwen Sun, Guoliang Li, Xingwang Li. The landscape of promoter-centered RNA-DNA interactions in rice. Nature Plants, 2022, 157–170.
2. Li Deng*, Baibai Gao*, Lun Zhao, Ying Zhang, Qing Zhang, Guoting Chen, Shuangqi Wang, Liang Xie, C. Robertson McClung, Guoliang Li, Xingwang Li. Diurnal RNAPII-tethered chromatin interactions are associated with rhythmic gene expression in rice. Genome Biology, 2022, 23: 7.
3. Weizhi Ouyang*, Shiping Luan*, Xu Xiang, Minrong Guo, Yan Zhang, Guoliang Li, Xingwang Li. Profiling Plant Histone Modification at Single-cell Resolution. Plant Biotechnology Journal, 2022, 20: 420–422.
4. Lun Zhao*, Shuangqi Wang*, Zhilin Cao*, Weizhi Ouyang, Qing Zhang, Liang Xie, Ruiqin Zheng, Minrong Guo, Meng Ma, Zhe Hu, Wing-Kin Sung, Qifa Zhang, Guoliang Li#, Xingwang Li#. Chromatin loops associated with active genes and heterochromatin shape rice genome architecture for transcriptional regulation. Nature Communications, 2019, 10: 3640.
5. Zhonghui Tang*, Oscar Luo*, Xingwang Li*, Meizhen Zheng, Jacqueline Jufen Zhu, Przemyslaw Szalaj, Pawel Trzaskoma, Adriana Magalska, Jakub Wlodarczyk, Blazej Ruszczycki, Paul Michalski, Emaly Piecuch, Ping Wang, Danjuan Wang, Simon Zhongyuan Tian, Xiaoan Ruan, May Penrad-Mobayed, Laurent M. Sachs, Chia-Lin Wei, Edison T. Liu, Grzegorz M. Wilczynski, Dariusz Plewczynski, Guoliang Li, Yijun Ruan. CTCF-mediated 3D genome architecture reveals a chromatin topology for transcription regulation. Cell, 2015, 163: 1611–1627.
6. YongPeng, DanXiong, LunZhao, WeizhiOuyang, ShuangqiWang, JunSun, QingZhang, PengpengGuan, LiangXie, WenqiangLi, GuoliangLi, JianbingYan, Xingwang Li, Chromatin interaction m aps reveal genetic regulation for quantitative traits in maize, Nature Communications, 2019,10: 2632
7. Weizhi Ouyang, DanXiong, Guoliang Li, Xingwang Li, Unraveling the 3D genome architecture in plants: present and future, Molecular Plant, 2020,13:1676-1693
8. WeizhiOuyang, QinXiao, GuoliangLi, Xingwang Li, Technologies for capturing 3D genome architecture in plants, Trends in Plant Science, 2021,26:196-197
9. LiangXie, MinghaoLiu, LunZhao, KaiCao, PengWang, WenhaoXu, Wing-KinSung, Xingwang Li, GuoliangLi, RiceENCODE: A comprehensive epigenomic database as a rice Encyclopedia of DNA Elements, Molecular Plant,2021,14:1604-1606
10. WeizhiOuyang, XiwenZhang, YongPeng, QingZhang, ZhilinCao, GuoliangLi, Xingwang Li, Rapid and low-input profiling of histone marks in plants using nucleus CUT&Tag, Frontiers in Plant Science,2021,12:634679
